Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Phenotypic Characterization of Collagen Induced Arthritis in DBA/1J Mice. DBA/1J mice were immunized with bovine type II collagen (N = 11–55; arthritic: n = 6–18; non-arthritic: n = 5–37) or PBS (N = 4–14). A) Representative photographs and E) 3D-rendered micro-CT radiographs of arthritic (red arrow = bone erosion), non-arthritic, and PBS-injected mouse hind limbs. Changes in B) clinical arthritis score, C) anti-bovine type II collagen (CII) IgG and D) anti-mouse CII IgG over time. Dashed line represents the antibody positivity cut-off, indicating the limit of detection. F) Hind limb bone mineral density and G) bone mineral density of bCII-immunized fore and hind limbs over time. H) Pain-like behaviour at day 47 post primary immunization. Graphs show the median [IQR], with each symbol in F-H) representing an individual mouse. For B-D), statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test, with resulting p-values shown in the graphs. Kruskal-Wallis with Dunn's multiple comparisons test was used for F) and H), and the resulting p-values were p = 0.0008 and p = 0.0003 respectively, with p-values for pairwise comparisons indicated on the graphs. For G), a Spearman correlation coefficient (r) was calculated and p < 0.05 was considered significant.

Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

Techniques: Micro-CT, Injection

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: Splenic T Cell Proliferation Following Stimulation with Type II Collagen Protein. Splenocytes isolated at day 49 post primary immunization from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 8; non-arthritic: N = 7) or PBS (N = 10) were cultured in media alone or 80 μg/mL bCII protein. Following 72 h incubation, A) CD4 + and B) CD8 + T cell proliferation following stimulation with bCII was measured by flow cytometry and is shown as a mean (±SD) stimulation index, with data points representing individual mice. Stimulation indices greater than 1.6 (dashed line) were considered a positive response. Statistical analysis was performed using one-way ANOVA with Tukey's multiple comparisons test, and the resulting p-values were p = 0.0054 and p = 0.0987 respectively, with p-values for pairwise comparisons indicated on the graphs. ns: not significant.

Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

Techniques: Isolation, Cell Culture, Incubation, Flow Cytometry

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

Article Snippet: Immunization-grade bovine type II collagen (bCII) protein was purchased from Chondrex Inc (20022; USA).

Techniques: Indirect ELISA

Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–C) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of apoptotic markers like Bax (A) , Bcl-2 (B) , and Caspase-3 (C) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–C) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: We assessed apoptotic markers, including Bcl-2 [KLR1880; Krishgen Biosystem, Mumbai, India], Bax [KLR0034; Krishgen Biosystem, Mumbai, India], and Caspase-3 [KLR1648; Krishgen Biosystem, Mumbai, India] ( ).

Techniques: Standard Deviation, Control

Journal: Renal Failure

Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

doi: 10.1080/0886022X.2026.2652103

Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB after renal I/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus DS group; # p < 0.05 versus DI/R group; ■ p < 0.05 versus DI/R + RSV group.

Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

Techniques: Expressing, Western Blot

Journal: Renal Failure

Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

doi: 10.1080/0886022X.2026.2652103

Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB in HK-2 cells after H/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus Control group; # p < 0.05 versus H/R group; ■ p < 0.05 versus H/R + RSV group.

Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

Techniques: Expressing, Western Blot, Control